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Autoclave

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Autoclave

Autoclave:  It is a double walled vessel made up of thick stainless steel or copper.  It is fitted with gauges which measure temperature and pressure, an exhaust valve (steam cock) for release of steam from the chamber, a safety valve to avoid accidents due to development of excess pressure, controls for adjusting temperature and pressure.  A rubber seal inside the lid maintains airtight condition.  Screw fasteners or fly nuts are used to secure the lid. Autoclave is used to sterilize all those substances that cannot be sterilized by dry heat such as, nutrient media, water, reagents, metabolites, instruments etc.

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